Infection may be referred to as duck septicaemia, goose flu, riemerellosis, new duck disease and polyserositis. Riemerella anatipestifer is a major pathogenic agent of duck septicemic and exudative diseases. Riemerella anatipestifer infections cause major economic losses in the duck industry. Comparative genomic analysis identifies structural. Unlimited viewing of the articlechapter pdf and any associated supplements and figures. Riemerella anatipestifer infection in domestic ducks in japan. It is a highly contagious bacterial disease that causes neurological, respiratory andor gastrointestinal signs in affected ducks. The results revealed that there were three different strains of ra involved. Riemerella anatipestifer is a welldescribed pathogenic bacterium, which is reported worldwide as the cause of epizootic infectious polyserositis of waterfowl and other avian species. Development and evaluation of a trivalent riemerella anatipestiferinactivated vaccine. Isolation and polymerase chain reactionbased identification. Find specific details on this topic and related topics from the msd vet manual. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript 2 editor. In this study, a trivalent inactivated vaccine of r.
Avian pathology 2002 31, 267270 a potential new serotype of riemerella anatipestifer isolated from ducks in thailand pornpen pathanasophon1, patchara phuektes1, tipa tanticharoenyos1, watcharachai narongsak1 and takuo sawada2 1 national institute of animal health, kasetklang, chatuchak, bangkok 10900, thailand, and 2nippon veterinary and animal science university, musashino, tokyo. Riemerella anatipestifer antibody indirect elisa method detection kit and application thereof. Identification and characterization of camp cohemolysin as. Ompa is a virulence factor of riemerella anatipestifer sciencedirect. Owing to concerns that the bacteria was being spread from farm to farm, an investigation using serotyping and dna fingerprinting was performed. The aim was to isolate and characterize riemerella anatipestifer organisms from disease outbreaks in ducks in kerala. Jul 02, 2016 the outbreak of riemerella anatipestifer ra infection has been confirmed in meattype domestic ducks anas platyrhynchos for the first time in 27 years in japan. Complete genome sequence of riemerella anatipestifer serotype 1. Find specific details on this topic and related topics from the merck vet manual. Genetic analyses suggest that this pathogen has a novel protein secretion system, known as the type ix secretion system t9ss. Comparative genomics of riemerella anatipestifer reveals genetic diversity comparative genomics of riemerella anatipestifer reveals genetic diversity.
Sep 17, 2018 in this study, the riemerella anatipestifer mutant strain ra1062 was obtained by screening a random tn4351 transposon mutant library. In this study, we evaluated the immune response and protective efficacy provided by a subunit vaccine containing recombinant outer membrane protein a rompa and plasmid constructs containing cpg oligodeoxynucleotides odn. Antimicrobial susceptibility of riemerella anatipestifer. Trivalent riemerella anatipestiferinactivated vaccine. Cn102323428a riemerella anatipestifer antibody indirect. Pdf on jan 1, 2004, rajeswari shome and others published an outbreak of riemerella anatipestifer infection in ducks in meghalaya find, read and cite all the research you need on researchgate. In this study, the cat gene was identified in 69192 36% r.
The outer membrane protein h omph of ra, expressed in li strain bl21, was first used as coating antigen to develop an indirect elisa assay for serological. Anatipestifer infection duckdvm diseases and conditions az. Riemerella anatipestifer ra, a major causative agent of septicemia anserum exsudativa in domesticated ducklings, has a protein secretion. Infection may be referred to as duck septicaemia, goose flu, riemerellosis, new. A commercial duck company that raises approximately two million pekin ducks per year experienced an outbreak of riemerella anatipestifer ra on nine farms over a 1yr period. A potential new serotype of riemerella anatipestifer isolated from ducks in thailand. Here, we present the complete genome sequence of r. Here, we investigated the transcriptomic effects of iron limitation. Riemerella anatipestifer is a pathogenic bacterium that has spread all over the world and is associated with epizootic infections in waterfowl and other avian species. Riemerella anatipestifer is a gramnegative, non motile, extracellular bacterium that belongs to the. Riemerella anatipestifer is a gramnegative, nonmotile, and nonsporeforming rodshaped bacterium. Epidemiology and antibiogram of riemerella anatipestifer isolated from waterfowl slaughterhouses in taiwan. Ermf and ered are responsible for erythromycin resistance. Antibiotic susceptibility of riemerella anatipestifer field.
Riemerella anatipestifer, a gramnegative bacillus, causes septicemia that can result in high mortality for ducklings. Development of a markerless gene deletion strategy using. Dec 19, 2017 riemerella anatipestifer ra, a major causative agent of septicemia anserum exsudativa in domesticated ducklings, has a protein secretion system known as the type ix secretion system t9ss. Jun 17, 2014 riemerella anatipestifer is one of the most important pathogens of ducks. The infection causes serious losses in ducks and geese due to high mortality. Pdf the outbreak of riemerella anatipestifer ra infection has been confirmed in meattype domestic ducks anas platyrhynchos for the first. It is encountered as acute or chronic septicaemia and is characterized with serous fibrinous polyserosites. Development and evaluation of a trivalent riemerella anatipestifer inactivated vaccine. Dec 01, 2009 four isolates were found to be resistant to 29 different antimicrobials. Riemerella anatipestifer gldm is required for bacterial. Riemerella anatipestifer infection in ducks induces il17a. Pdf complete genome sequence of riemerella anatipestifer.
However, the genes involved in iron metabolism and the mechanisms of iron transport are largely unknown. Mar 11, 2020 riemerella anatipestifer infection causes serious economic losses in the duck industry worldwide. Ermf and ered are responsible for erythromycin resistance in riemerella anatipestifer, plos one, 2015. Riemerella anatipestifer causes serositis and septicaemia in domestic ducks, geese, and turkeys. In a previous study, we established a markerless mutation system based on the phes mutant as a counterselectable marker. To answer this question, we constructed an ra mutant deficient in sprt, which. Comparative genomics of riemerella anatipestifer reveals genetic diversity, bmc genomics, 2014, pp. Riemerella anatipestifer ra is a gramnegative bacterium in the family flavobacteriaceae and rrna superfamily v. Riemerella anatipestifer is sick, and riemerellla anatipestifer infection rai is a kind of social disease of tame duck, turkey and multiple other birds. Rifampin resistance and its fitness cost in riemerella anatipestifer. Riemerella anatipestifer is responsible for an economically important disease of commercially raised ducks. Riemerella anatipestifer is a member of the flavobacteriaceae family.
Epidemiologic investigation of riemerella anatipestifer in a. Riemerella anatipestifer is an infectious pathogen causing serositis in ducks. Several outbreaks of ra infection occurred worldwide, particularly in the duck 2,3. It causes a contagious septicemic disease in domesticated ducklings, turkeys, and various other birds and accounts for major economic losses in industrialized duck production due to weight loss, high mortality, and culling. Antibiotic susceptibility of riemerella anatipestifer. In this study, we present the complete genomes of two r. Development of a subunit vaccine containing recombinant. Frontiers riemerella anatipestifer type ix secretion. It is a gramnegative bacterium that causes septicaemia and death in young ducks and geese throughout the world.
Although more and more genomes of ra have been generated comparaed to genomic analysis of ra still remains at the level of individual species. The synonymous codon usage patterns of bacteria reflect a series of evolutionary changes that enable bacteria to improve tolerance of the various environments. Anatipestifer infection, otherwise known as new duck disease and duck septicaemia, is caused by riemerella anatipestifer previously referred to as pasteurella anatipestifer bacteria. Isolation and polymerase chain reactionbased identification of riemerella anatipestifer from ducks in kerala, india, veterinary world 79. Riemerella anatipestifer is a major bacterial pathogen that causes septicemic and exudative diseases in domestic ducks. Ermf and ered are responsible for erythromycin resistance in riemerella anatipestifer. Ulrike gertrud munderloh, university of minnesota, united states to investigate the genetic basis of erythromycin resistance in riemerella anatipestifer, the mic to erythromycin of 79 r. Riemerella anatipestifer ra infection is a contagious disease in domestic ducks, turkeys and other fowl. Evaluation of the protective immunity of riemerella anatipestifer ompa. Riemerella anatipestifer is a gramnegative bacterium that mainly infects ducks, turkeys and other birds. Trivalent riemerella anatipestifer inactivated vaccine. Development and evaluation of a trivalent riemerella. Genome sequence of avirulent riemerella anatipestifer. The bacteria is often spread between birds by mucus that has been exhaled.
Riemerella anatipestifer is one of the most important pathogens of ducks. Overview of riemerella anatipestifer infection in poultry. The genome sequence of this species is presented herein. Riemerella anatipestifer is a gram negative bacterial pathogen primarily causing septicaemia in ducks and geese. Pdf ermf and ered are responsible for erythromycin. Comparative genomics of riemerella anatipestifer reveals.
Although more and more genomes of ra have been generated comparaed to genomic analysis of ra. The diseased birds suffer from respiratory and nervous symptoms and during necropsy, the dead birds had pericarditis, perihepatitis, and meningitis. Riemerella anatipestifer is a causative pathogen of diseases in ducks, geese, turkeys, and various other domestic and wild birds. To investigate the genetic basis of erythromycin resistance in riemerella. Frontiers type b chloramphenicol acetyltransferases are. The ducklings at the age of 18 weeks are especially susceptible. Characterization of the hemolytic activity of riemerella. In january 2014, increased mortality in a 14 to 21dayold duck flock was reported to veterinary officials by the owner.
Dietary supplementation of bacillus subtilis probiotics. Animal experiments showed that the ducks that received two immunizations with the vaccine were 100% protected from challenge with strains from any of. Download pdf abstractexcerpt pdf references riemerella anatipestifer causes anatipestifer disease in many avian species. Utility of molecular biology tools for identification and characterization of egyptian riemerella anatipestifer duck isolates.
Riemerella anatipestifer ra is a grams negative bacteria that cause respiratory disease in ducks, geese, turkey and chicken. Epidemiology and antibiogram of riemerella anatipestifer. Complete genome sequence of riemerella anatipestifer type. It is unknown whether the t9ss contributes to the virulence of ra through secretion of factors associated with pathogenesis. One of the important elements for most bacterial growth is iron, the bioavailability of which is limited in hosts. Epidemiologic investigation of riemerella anatipestifer in. The associated disease represents a major problem for the duck industry worldwide, and is the cause of significant economic losses. Acute septicemia and high blood bacterial loading in r. Epidemiology and antibiogram of riemerella anatipestifer isolated. Original article development of an indirect elisa using. Infected ducks show clinical signs of lethargy, diarrhea, and respiratory and nervous symptoms, all of which cause serious economic losses in the duck industry.
Traditionally, the antibiotics were used to treat this disease. Recombinant ompa displayed a molecular mass similar to that predicted from the nucleotide sequence of the ompa gene but lower than that observed in total cell lysates of r. Characterization of the riemerella anatipestifer m949. Pdf development and evaluation of a trivalent riemerella. Riemerella anatipestifer ra belongs to the flavobacteriaceae family and can cause a septicemia disease in poultry. Whilst we tend to think of ducks as being bulletproof in terms of disease there are a few deadly conditions that can infect them. Complete genome sequence of riemerella anatipestifer serotype 10 strain hxb2. If the inline pdf is not rendering correctly, you can download the pdf file here. Bmc genomics comparative genomics of riemerella anatipestifer reveals genetic diversity xiaojia wang 0 2 wenbin liu 3 dekang zhu 2 5 linfeng yang 3 mafeng liu 0 2 5 sanjun yin 3 mingshu wang 0 2 5 renyong jia 0 2 5 shun chen 0 2 5 kunfeng sun 0 2 5 anchun cheng 0 2 5 xiaoyue chen 2 5 0 institute of preventive veterinary medicine, sichuan agricultural university, chengdu, sichuan 611, p.
Genetic variation of the ompa and 16s rrna genes of riemerella. The outbreak of riemerella anatipestifer ra infection has been confirmed in meattype domestic ducks anas platyrhynchos for the first time in 27 years in japan. Riemerella anatipestifer is an important pathogen of waterfowl, which causes septicemia anserum exsudativa in ducks. There are 21 known serotypes and infection is spread horizontally between birds.
Pdf a potential new serotype of riemerella anatipestifer. Therefore, the immunogenicity induced by a broth culture bacterin prepared from a local strain 1081 of this serotype was studied in ducks and a protective index pi was derived. Pdf riemerella anatipestifer infection in domestic ducks in japan. It usually occurs in young birds of 18 weeks of age.
Isolation and identification of riemerella anatipestifer from ducks in malaysia 23 r. The completed draft genome consists of one circular chromosome with 2,164,087 bp. In particular, the lack of genomic information from a variety of r. Riemerella anatipestifer is a gramnegative, nonmotile, nonsporeforming, rodshaped bacterium belonging to the flavobacteriaceae family in rrna superfamily v. Riemerella anatipestifer infection causes serious economic losses in the duck industry worldwide. The disease is commonly referred as new duck disease. There are 2,101 genes in the draft, and its gc content is 35. Ermf and ered are responsible for erythromycin resistance in. Immunogenicity of riemerella anatipestifer broth culture. Introduction riemerella anatipestifer is a major bacterial pathogen of avian species, causing severe economic loss in commercially important poultry worldwide. Learn about the veterinary topic of overview of riemerella anatipestifer infection in poultry. Complete genome sequence of riemerella anatipestifer. Riemerella anatipestifer infection is a serious worldwide problem of natural waterfowl and. Epidemiology and antibiogram of riemerella anatipestifer isolated from waterfowl slaughterhouses in.
To investigate the genetic basis of erythromycin resistance in riemerella anatipestifer, the mic to erythromycin of 79 r. We detailed the codon usage patterns of ra isolates from the available 12 sequenced genomes by multiple codon and. Riemerella anatipestifer ra, the causative agent of duck septicemia, results in high mortality of ducklings and causes great economic losses in duck raising industry. Strains belonging to riemerella anatipestifer serotype 1 are responsible for most of the major disease outbreaks caused by this organism in ducks in thailand. Riemerella anatipestifer drug resistance profiles changed over time, and the only consistent patterns observed were the resistance of r. Characterization of a predominant immunogenic outer. Riemerella anatipestifer ra, a major causative agent of septicemia anserum exsudativa in domesticated ducklings, has a protein secretion system known as the type ix secretion system t9ss. The present invention relates to the fast diagnosis method and the utensil of a kind of bacterial antibodies of duck, is a kind of riemerella anatipestifer antibody indirect elisa method detection kit and application thereof more specifically. Isolation and identification of riemerella anatipestifer. Riemerella anatipestifer is a gramnegative, nonmotile, nonspore forming, and atrichous bacterium belonging to the family flavobacteriaceae in the phylum bacteroidetes.
Riemerella anatipestifer is a gramnegative, nonsporeforming, rodshaped bacterium that mainly affects commerciallyimportant birds such as ducks and turkeys 1,2. Riemerella anatipestifer type ix secretion system is. Jun 01, 2010 a commercial duck company that raises approximately two million pekin ducks per year experienced an outbreak of riemerella anatipestifer ra on nine farms over a 1yr period. The ompa gene, encoding the 42kda major antigenic outer membrane protein ompa of riemerella anatipestifer, the etiololgical agent of septicemia anserum exsudativa, was cloned and expressed in escherichia coli. There are 21 known serotypes with no crossreactivity between them. A potential new serotype of riemerella anatipestifer. Frontiers riemerella anatipestifer type ix secretion system. Subsequently, pmeg375lsr was further transformed into e.
A potential new serotype of riemerella anatipestifer isolated. Riemerella anatipestifer causes anatipestifer disease in many avian species. Transmission between ducks occurs vertically through the egg as well as horizontally via the respiratory tract. Riemerella anatipestifer infection is a septicaemic acute, peracute, sometimes chronic disease of primarily ducks and geese, but it also affects turkeys, chickens, pheasants, guinea fowls, quails and swans. When cultured in trypticase soy broth, the late stage growth of the. Nov 12, 2007 strains belonging to riemerella anatipestifer serotype 1 are responsible for most of the major disease outbreaks caused by this organism in ducks in thailand. Free fulltext pdf articles from hundreds of disciplines, all in one place. Pdf an outbreak of riemerella anatipestifer infection in ducks in. The affected ducks exhibited reduced movement, ataxia and dorsal recumbency with leg paddling. The disease is caused by an organism related to pasteurella called riemerella anatipestifer.
356 1471 1188 1167 587 723 1349 572 1405 952 897 988 614 324 50 180 771 1264 245 1472 671 926 1035 947 924 255 1210 1367 1203 1529 963 1521 895 1346 650 1476 857 1306 207 1007 7 423 1012 956